Cyno CD28 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-263 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable CHOK1 cell line expressing exogenous cyno CD28 gene
Application
Drug screening and biological assays
Background
CD28, also known as TP44, is a single-pass type I membrane protein. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 and CD86. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells. The CD86 expression on antigen-presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Product Information
| Species |
Mouse |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI1640 + 10% FBS + 10 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:8. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 24 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
