| Catalog Number | CBCL-263 |
| Price | Online Inquiry |
| Unit Quantity | 1x106 cells / 1.0 ml |
Stable CHOK1 cell line expressing exogenous cyno CD28 gene
Drug screening and biological assays
CD28, also known as TP44, is a single-pass type I membrane protein. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 and CD86. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells. The CD86 expression on antigen-presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
| Category | Immune Molecules Cell Lines |
| Cell Line Name | Cyno CD28 Stable CHOK1 Cell Line |
| Pharmaceutical Target | Immune Molecules |
| Species | Hamster |
| Host Cell Line | CHOK1 |
| Cell Type | Human embryonic kidney cells |
| Morphology | Monolayer |
| Growth Properties | Adherent |
| Selection Marker | Puromycin |
| Seeding Density | Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio | Recommended a ratio of 1:4-1:8. |
| Doubling Time | Approximately 24 hours |
| Recommended Medium | RPMI1640 + 10% FBS + 10 μg/ml Puromycin |
| Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability | Stable in culture over 10 passages |
| QC | WB |
| Shipping | Shipping in dry ice |
| Biosafety Level | BSL-1 |
| Caution | For Research Use Only |