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Cyno CD28 Stable CHOK1 Cell Line

Catalog Number CBCL-263
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable CHOK1 cell line expressing exogenous cyno CD28 gene

Application

Drug screening and biological assays

Background

CD28, also known as TP44, is a single-pass type I membrane protein. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 and CD86. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells. The CD86 expression on antigen-presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name Cyno CD28 Stable CHOK1 Cell Line
Pharmaceutical Target Immune Molecules
Species Hamster
Host Cell Line CHOK1
Cell Type Human embryonic kidney cells
Morphology Monolayer
Growth Properties Adherent
Selection Marker Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:8.
Doubling Time Approximately 24 hours
Recommended Medium RPMI1640 + 10% FBS + 10 μg/ml Puromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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