Cyno CD47 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-544 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
The CHO-K1-Cyno CD47-Stable Cell Line consists of recombinant CHO-K1 cells that stably overexpress cynomolgus CD47 on their surface. The surface expression of CD47 is confirmed by FACS analysis.
Application
Drug screening and biological assays
Background
CD47, also named as integrin associated protein (IAP) is a transmembrane protein belonging to the immunoglobulin superfamily, which acts as a signal to macrophage after binding with its ligand signal-regulatory protein alpha (SIRPa), and is potential therapeutic target in some cancer and treatment of pulmonary fibrosis.
Product Information
| Category |
Immune Checkpoint Cell Lines |
| Cell Line Name |
CHO-K1-Cyno CD47-Stable Cell Line |
| Pharmaceutical Target |
Immune Molecules |
| Protein Family |
Protein family of scavenger receptors |
| Gene |
CD47 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:8. |
| Doubling Time |
Approximately 24 hours |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin |
| Freeze Medium |
95% complete growth medium + 5% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| QC |
WB |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
