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Cyno CD73 Stable CHOK1 Cell Line

Catalog Number CBCL-354
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable CHOK1 cell line expressing exogenous human INSR gene

Application

Drug screening and biological assays

Background

CD73 is a glycosylphosphatidylinositol (GPI) anchored cell surface protein that is encoded by NTSE gene, which plays a crucial role in switch on adenosinergic signaling. CD73 is highly expressed in breast cancer, colorectal cancer, ovarian cancer, gastric cancer, and gallbladder cancer and associated. This gene can also be used asa key regulatory molecule in cancer development.

Product Information

Species Mouse
Host Cell Line CHOK1
Cell Type Chinese hamster ovary cell line
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium F12K + 10% FBS + 10 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:8.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 24 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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