Cyno CTLA4 Stable 293T Cell Line

Catalog Number	CBCL-595
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable 293T cell line expressing exogenous cyno CTLA4 gene

Application

Drug screening and biological assays

Background

CTLA4, also known as CD152, is a protein receptor that belongs to the immunoglobulin superfamily, which is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Cyno CTLA4 Stable 293T Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Morphology	Epithelial
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 30 hours
Recommended Medium	DMEM + 10% FBS + 1 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.