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Cyno TIGIT Stable 293T Cell Line

Catalog Number CBCL-403
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HEK293T cell line expressing exogenous monkey TIGIT gene

Application

Drug screening and biological assays

Background

TIGIT (T cell Immunoreceptor with Ig and ITIM domains) is a type I transmembrane protein belonging to B7-CD28 superfamily. TIGIT is mainly expressed on NK cells and activated, memory and regulatory T cells. It can bind to CD155 and CD122, and play an important role in T cell activation and susceptibility to autoimmune challenges.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 1 μg/ml Puromycin
Selection Marker Puromycin and hygromycin B
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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