EGFR T790M/C797S/L858R overexpressed Stable NCI-H1975 Cell Line

Catalog Number	CBCL-438
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

NCI-H1975 cell line bearing exogenous EGFR T790M/C797S/L858R mutations

Application

Drug screening and biological assays

Background

EGFR, the epidermal growth factor receptor, is a cell-surface receptor tyrosine kinase, and activated by binding of its specific ligand, such as epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The overexpression or overactivity of EGFR has associated with a number of cancers, including the lung cancer and colon cancer.

Product Information

Species	Human
Host Cell Line	NCI-H1975
Cell Type	Human non-small cell lung cancer cell line
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	RPMI 1640 + 10% FBS + 2 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:3-1:6.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.