EGFR T790M/C797S/ L858R Stable NCI-H1975 Cell Line

Catalog Number	CBCL-431
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable NCI-H1975 cell clone bearing endogenous EGFR C797S mutations

Application

Drug screening and biological assays

Background

EGFR, the epidermal growth factor receptor, is a cell-surface receptor tyrosine kinase, and activated by binding of its specific ligand, such as epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The overexpression or overactivity of EGFR has associated with a number of cancers, including the lung cancer and colon cancer.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	EGFR T790M/C797S/ L858R Stable NCI-H1975 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	NCI-H1975
Cell Type	Human non-small cell lung cancer cell line
Morphology	Epithelial
Growth Properties	Adherent
Selection Marker	Hygromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:3-1:6.
Recommended Medium	RPMI 1640 + 10% FBS + 100 μg/ml Hygromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.