Catalog Number | CBCL-220 |
Price | Online Inquiry |
Unit Quantity | 1x106 cells / 1.0 ml |
Stable Ba/F3 clone expressing exogenous ETV6-NTRK3 fusion protein bearing F617L mutation in NTRK3 part.
Drug screening and biological assays
NTRK3, also named Tropomyosin receptor kinase C (TrkC), is the high affinity catalytic receptor for the neurotrophy and play a major role in neuronal differentiation and survival, was originally found in congenital fibrosarcoma and subsequently found in secretory breast cancer and other type cancer, the identification of NTRK1 fusion genes as driver genes has led to the rapid development of anticancer therapeutics agents. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Category | Kinase Cell Lines |
Cell Line Name | ETV6-NTRK3 F617L Stable Ba/F3 Cell Line |
Pharmaceutical Target | Kinases |
Species | Mouse |
Host Cell Line | Ba/F3 |
Cell Type | Pro-B cells |
Morphology | Mostly round |
Growth Properties | Suspension |
Selection Marker | Puromycin |
Seeding Density | Thaw entire contents into an appropriate T25 flask. |
Subculture Ratio | Recommended a ratio of 1:10. |
Doubling Time | Approximately 20 hours |
Recommended Medium | RPMI 1640 + 10% FBS |
Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
Stability | Stable in culture over 10 passages |
QC | WB |
Shipping | Shipping in dry ice |
Biosafety Level | BSL-1 |
Caution | For Research Use Only |