Contact Us

USA

Tel:
Fax:
Email:
Europe
Tel:
Email:

;

FIP1L1-PDGFRA-D842V Stable Ba/F3 Cell Line

Catalog Number CBCL-188
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable Ba/F3 clone expressing exogenous FIP1L1-PDGFRA fusion protein bearing D842V mutation in PDGFRA part.

Application

Drug screening and biological assays

Background

Platelet-derived growth factor receptor A, abbreviated as PDGFRA or PDGFRa, is a cell-surface receptor tyrosine kinase, and activated by binding of its specific ligand, platelet-derived growth factors (PDGFs), play an important role in cell growth and differentiation. The identification of PDGFRA as a driver gene has led to the development of anticancer therapeutics agents. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.

Product Information

Species Mouse
Host Cell Line Ba/F3 cell line
Cell Type Pro-B cells
Stability Stable in culture over 10 passages
Growth Properties Suspension
Pharmaceutical Target Kinases
Recommended Medium RPMI 1640 + 10% FBS
Selection Marker Puromycin
Morphology Mostly round
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
Copyright © 2024 Creative Bioarray. All rights reserved.