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FLT3-ITD-A848P Stable Ba/F3 Cell Line

Catalog Number CBCL-155
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable Ba/F3 clone expressing exogenous FLT3 bearing ITD duplicate and A848P amino acid double mutations.

Application

Drug screening and biological assays

Background

FMS-like tyrosine kinase 3 (FLT3) is a receptor tyrosine kinase that is expressed almost exclusively in the hematopoietic compartment. Its ligand, FLT3 ligand (FL), induces dimerization and activation of its intrinsic tyrosine kinase activity. The identification of FLT3 as a driver gene has led to the repaid development of anti-AML therapeutics. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.

Product Information

Species Mouse
Host Cell Line Ba/F3 cell line
Cell Type Pro-B cells
Stability Stable in culture over 10 passages
Growth Properties Suspension
Pharmaceutical Target Kinases
Recommended Medium RPMI 1640 + 10% FBS
Selection Marker Puromycin
Morphology Mostly round
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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