FLT3-ITD-D835F Stable Ba/F3 Cell Line
| Catalog Number |
CBCL-157 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable Ba/F3 clone expressing exogenous FLT3 bearing ITD duplicate and D835F amino acid double mutations.
Application
Drug screening and biological assays
Background
FMS-like tyrosine kinase 3 (FLT3) is a receptor tyrosine kinase that is expressed almost exclusively in the hematopoietic compartment. Its ligand, FLT3 ligand (FL), induces dimerization and activation of its intrinsic tyrosine kinase activity. The identification of FLT3 as a driver gene has led to the repaid development of anti-AML therapeutics.
Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Product Information
| Category |
Kinase Cell Lines |
| Cell Line Name |
FLT3-ITD-D835F Stable Ba/F3 Cell Line |
| Pharmaceutical Target |
Kinases |
| Species |
Mouse |
| Host Cell Line |
Ba/F3 |
| Cell Type |
Pro-B cells |
| Morphology |
Mostly round |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:10. |
| Doubling Time |
Approximately 20 hours |
| Recommended Medium |
RPMI 1640 + 10% FBS |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability |
Stable in culture over 10 passages |
| QC |
WB |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
