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GUCY2C Stable 293T Cell Line

Catalog Number CBCL-253
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable 293T cell line expressing exogenous human GUCY2C gene.

Application

Drug screening and biological assays

Background

GUCY2C, also known as GC-C, is a type | transmembrane protein of the guanylate cyclase (GC) family. GUCY2C and its hormones guanylin and progranulin have emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Kinases
Recommended Medium DMEM + 10% FBS + 1 μg/ml Puromycin
Selection Marker Puromycin
Morphology Epithelial
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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