HEK293-FcRn (FCGRT & B2M), GFP Tag-Stable Cell Line
| Catalog Number |
CBCL-0025 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The HEK293/FcRn (FCGRT & B2M) Stable Cell Line was engineered to stably express exogenous human FcRn (FCGRT&B2M), with FCGRT (AA sequence: Ala 24 - Ala 365, Protein Acc.#: P55899-1) and B2M (AA sequence: Ile 21 - Met 119, Protein Acc.#: P61769-1). Concurrently, GFP was expressed in these cells as a marker for screening positive cells via FACS.
Application
Binding assay by FACS and cell based ELISA.
Background
The FCGRT gene encodes the neonatal Fc receptor (FcRn), which forms a heterodimer with β2-microglobulin (encoded by the B2M gene). FcRn is expressed in various tissues, including the placenta, intestine, and endothelial cells. It plays a critical role in the transport and protection of IgG across epithelial barriers and in the placenta. FcRn binds to IgG at acidic pH in endosomes and releases it at neutral pH, allowing for the recycling and long half-life of IgG in the circulation. It also participates in the presentation of antigens to immune cells.
Product Information
| Category |
Fc Receptor Cell Lines |
| Cell Line Name |
HEK293-FcRn (FCGRT & B2M), GFP Tag-Stable Cell Line |
| Protein Family |
Neonatal Fc receptor |
| Gene |
FcRn/FCGRT & B2M |
| NCBI Gene ID |
9592 (FCGRT)
567 (B4M) |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Zeocin (25 μg/mL) + Puromycin (5 μg/mL) |
| Recommended Medium |
DMEM medium +10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
