HEK293-GIPR/CRE-Luciferase-Reporter Cell Line
| Catalog Number |
CBCL-0218 |
| Price |
Online Inquiry |
| Unit Quantity |
2 x 10^6 cells / 1.0 ml |
Introduction
Recombinant HEK293 cells expressing the firefly luciferase gene under the control of the cAMP response element (CRE), with forced expression of human GIPR (Gastric Inhibitory Polypeptide receptor, NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity. The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated using agonists gastric inhibitory peptide (GIP) and tirzepatide hydrochloride, which induce luciferase activity in a dose-dependent manner.
Application
Screen and characterize agonists of human GIPR in a cellular context
Background
GIPR (Gastric Inhibitory Polypeptide Receptor) is a G protein-coupled receptor that binds GIP and regulates insulin secretion and glucose homeostasis.
Product Information
| Category |
CRE Reporter Cell Lines |
| Cell Line Name |
HEK293-GIPR/CRE-Luciferase-Reporter Cell Line |
| Protein Family |
G protein-coupled receptor family |
| Gene |
GIPR |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
90% complete medium + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
