HEK293-Human 4-1BB (Luc)-Reporter Cell
| Catalog Number |
CBCL-0569 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The HEK293-Human 4-1BB (Luc)-Reporter Cell is engineered to express both the NF-KB signaling response element and the receptor full-length human 4-1BB (Uniprot: Q07011), which can drive luciferase expression systems upon 4-1BB ligand/agonist antibody stimulation. In the absence of agonist antibody or 4-1BB ligand, the 4-1BB receptor is not activated, and luminescence signal is low.
Application
Screen for ligands or agonist antibodies that can bind and activate 4-1BB
Background
4-1BB (TNFRSF9, CD137) is a co-stimulatory receptor expressed on activated T cells, NK cells, and dendritic cells. It binds to 4-1BB Ligand (TNFSF9) on APCs, promoting T cell proliferation, survival, and cytokine production. 4-1BB agonists are being tested in cancer immunotherapy.
Product Information
| Category |
Immune Checkpoint Cell Lines |
| Cell Line Name |
HEK293-Human 4-1BB (Luc)-Reporter Cell |
| Protein Family |
TNF receptor superfamily |
| Gene |
4-1BB |
| NCBI Gene ID |
3604 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
