Introduction
A recombinant HEK293 cell line engineered to express the human 5-HT1A receptor fused with a luciferase reporter gene. This cell line is designed to monitor the activation of the 5-HT1A receptor through changes in luciferase activity, allowing for the study of ligand binding and downstream signaling pathways.
Background
The HTR1A gene encodes the 5-hydroxytryptamine (serotonin) receptor 1A, a G protein-coupled receptor. It is widely distributed in the central nervous system, including the hippocampus, cortex, and raphe nuclei. This receptor is involved in regulating mood, anxiety, sleep, and cognitive function. Activation of 5-HT1A receptors has anxiolytic and antidepressant effects, while dysfunction of these receptors is associated with various psychiatric disorders, such as depression, anxiety disorders, and schizophrenia.
