HEK293-Human 5-HT1A (Luc)-Reporter Cell
| Catalog Number |
CBCL-0228 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human 5-HT1A (Luc) HEK293 Reporter Cell was engineered to express the CREB signaling response element, the full-length human 5-HT1A receptor (Uniprot: P08908), and mGFP using a viral P2A self-cleaving peptide. Stimulation with a human 5-HT1A agonist can drive CREB-mediated luminescence. Inhibition of activated 5-HT1A signaling by corresponding inhibitors results in decreased luminescence.
Application
Screen for 5-HT1A agonist.
Background
5-HT1A (also known as HTR1A) is a serotonin receptor subtype involved in regulating mood, anxiety, and cognitive functions. It is a target for antidepressant and anxiolytic drugs.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human 5-HT1A (Luc)-Reporter Cell |
| Protein Family |
5-Hydroxytryptamine receptor |
| Gene |
5-HT1A/HTR1A |
| NCBI Gene ID |
3350 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
