HEK293-Human CCR5-Stable Cell Line
| Catalog Number |
CBCL-0027 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A stable HEK293 cell line expressing the human CCR5 receptor. This cell line is useful for studying the interactions of CCR5 with its ligands, particularly in the context of HIV-1 infection and chemotaxis. The stable expression of CCR5 allows for consistent and reliable experimental results.
Application
Binding assay by FACS and cell based ELISA.
Background
The CCR5 gene encodes a chemokine receptor that belongs to the G protein-coupled receptor family. It is expressed on the surface of T cells, macrophages, and dendritic cells. CCR5 serves as a co-receptor for the entry of human immunodeficiency virus type 1 (HIV-1) into host cells. Individuals with a deletion mutation in the CCR5 gene (CCR5-Δ32) are resistant to HIV-1 infection. CCR5 also plays a role in the migration of immune cells to sites of inflammation and in the regulation of immune responses.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
HEK293-Human CCR5-Stable Cell Line |
| Protein Family |
Chemokine receptor |
| Gene |
CCR5 |
| NCBI Gene ID |
1234 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (5 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
10% DMSO + 90% FBS |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
