HEK293-Human GIPR (Luc)-Reporter Cell
| Catalog Number |
CBCL-0245 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This cell line is a HEK293-based reporter cell line engineered to express the CREB signaling response element and the full-length human GIPR (Uniprot: P48546-1). It can drive luciferase expression systems through GIPR agonists or Glucose-dependent insulinotropic polypeptide (GIP) stimulation. In the absence of agonist or GIP, the GIPR receptor is not activated and luminescence signal is low. In the presence of agonist or GIP, the GIPR pathway-activated luminescence can be detected in a dose-dependent manner.
Application
Screen for agonists that can bind and activate GIPR.
Background
GIPR (Gastric Inhibitory Polypeptide Receptor) is a G protein-coupled receptor that binds GIP and regulates insulin secretion and glucose homeostasis.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human GIPR (Luc)-Reporter Cell |
| Protein Family |
G protein-coupled receptor family |
| Gene |
GIPR |
| NCBI Gene ID |
2696 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
