HEK293-Human GLP-1R (Luc)-Reporter Cell
| Catalog Number |
CBCL-0037 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A recombinant HEK293 cell line expressing the human glucagon-like peptide-1 receptor (GLP-1R) fused with a luciferase reporter gene. This cell line is used to study the activation of the GLP-1R receptor and its downstream signaling pathways through luciferase activity.
Application
Screen for agonists that can bind and activate GLP-1R
Background
The GLP-1R gene encodes the glucagon-like peptide-1 (GLP-1) receptor, a G protein-coupled receptor. It is expressed in pancreatic beta cells, the brain, and the gastrointestinal tract. GLP-1 is secreted by the intestinal L cells in response to food intake and binds to its receptor on beta cells, stimulating insulin secretion in a glucose-dependent manner. GLP-1 also inhibits glucagon secretion, slows gastric emptying, and promotes satiety. GLP-1 receptor agonists are used as drugs for the treatment of type 2 diabetes and obesity.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
HEK293-Human GLP-1R (Luc)-Reporter Cell |
| Protein Family |
Class B G-protein-coupled receptor |
| Gene |
GLP-1R |
| NCBI Gene ID |
2740 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
