HEK293-Human HVEM (Luc)-Reporter Cell
| Catalog Number |
CBCL-0247 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This cell line is a HEK293-based reporter cell line engineered to express the NF-kB signaling response element and the full-length human HVEM (Uniprot: Q92956-1). When co-cultured with target cells expressing human BTLA, the BTLA/HVEM interaction drives NF-kB-mediated luminescence. Blocking the BTLA/HVEM interaction by either anti-BTLA or anti-HVEM antibodies results in a decrease in luminescence.
Application
Screen for anti-human BTLA antibody or anti-human HVEM antibody blocking BTLA/HVEM interaction.
Background
HVEM (Herpesvirus Entry Mediator) is a tumor necrosis factor receptor superfamily member that interacts with ligands such as BTLA and LIGHT to regulate immune responses.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human HVEM (Luc)-Reporter Cell |
| Protein Family |
Tumor necrosis factor receptor superfamily |
| Gene |
HVEM |
| NCBI Gene ID |
8764 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
