HEK293-Human HVEM (Luc)-Reporter Cell
| Catalog Number |
CBCL-0578 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The HEK293-Human HVEM (Luc)-Reporter Cell is engineered to express both the NF-kB signaling response element and the receptor full-length human HVEM (Uniprot: Q92956-1). When co-cultured with target cells expressing human BTLA, the BTLA/HVEM interaction drives NF-kB-mediated luminescence. Blocking the BTLA/HVEM interaction by either anti-BTLA or anti-HVEM antibodies results in a decrease in luminescence.
Application
Screen for anti-human BTLA antibody or anti-human HVEM antibody blocking BTLA/HVEM interaction.
Background
HVEM (Herpesvirus Entry Mediator, TNFRSF14) is a dual-function receptor expressed on immune cells. It binds to LIGHT (TNFSF14, activating) or BTLA/CD160 (inhibitory), modulating lymphocyte activation and immune tolerance. HVEM is involved in autoimmune diseases and tumor immune evasion.
Product Information
| Category |
Immune Checkpoint Cell Lines |
| Cell Line Name |
HEK293-Human HVEM (Luc)-Reporter Cell |
| Protein Family |
TNF receptor superfamily |
| Gene |
HVEM |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
