HEK293-Human IGF-1 R (Luc)-Reporter Cell
| Catalog Number |
CBCL-0248 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This cell line is a HEK293-based reporter cell line engineered to express a signaling response element and the full-length human IGF-1 R (Uniprot: P08069). When stimulated with human IGF-1 protein, the IGF-1/IGF-1 R interaction drives RE-mediated luminescence. Neutralization of the biological effect of human IGF-1 protein by corresponding antibodies results in a decrease in luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human IGF-1 protein.
Background
IGF-1 R (Insulin-like Growth Factor 1 Receptor) is a receptor tyrosine kinase that binds IGF-1 and regulates cell growth, proliferation, and survival.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human IGF-1 R (Luc)-Reporter Cell |
| Protein Family |
Insulin receptor family |
| Gene |
IGF-1 R |
| NCBI Gene ID |
3480 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
