HEK293-Human IL-1 R1 & IL-1 RAcP (Luc)-Reporter Cell
| Catalog Number |
CBCL-0198 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human IL-1 R1 & IL-1 RAcP (Luc) HEK293 Reporter Cell has been designed to express the NF-κB signaling response element. Stimulation with human IL-1 alpha or IL-1 beta protein triggers receptor-mediated signaling, resulting in NF-κB-mediated luminescence. Neutralizing the biological effect of the ligand-receptor interaction with corresponding antibodies decreases luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human IL-1 alpha or IL-1 beta protein
Background
IL - 1 R1 (Interleukin 1 Receptor Type 1) is a cell - surface receptor that binds interleukin - 1 (IL - 1) cytokines. IL - 1 RAcP (Interleukin 1 Receptor Accessory Protein) is a co - receptor that associates with IL - 1 R1 to form a functional signaling complex, mediating the biological effects of IL - 1 such as inflammation, immune response, and fever.
Product Information
| Category |
Cytokine Receptor Cell Lines |
| Cell Line Name |
HEK293-Human IL-1 R1 & IL-1 RAcP (Luc)-Reporter Cell |
| Protein Family |
Interleukin receptor family |
| Gene |
IL-1 R1 & IL-1 RAcP |
| NCBI Gene ID |
IL - 1 R1: 3554
IL - 1 RAcP: 3556 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
