HEK293-Human IL-10 R alpha/IL-10 R beta (Luc)-Reporter Cell
| Catalog Number |
CBCL-0199 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell has been engineered to express the STAT3 signaling response element, as well as the full-length human IL-10 R alpha (Uniprot: Q13651) and IL-10 R beta (Uniprot: Q08334) receptors. Stimulation with human IL-10 protein induces the IL-10 R alpha/IL-10 R beta interaction, driving STAT3-mediated luminescence. Neutralization of the biological effect of human IL-10 protein with corresponding antibodies reduces luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human IL-10 protein
Background
IL - 10 R alpha and IL - 10 R beta are subunits of the interleukin - 10 receptor. They form a heterodimeric receptor complex that binds IL - 10, a cytokine with anti - inflammatory and immunosuppressive properties, regulating immune responses and maintaining immune homeostasis.
Product Information
| Category |
Cytokine Receptor Cell Lines |
| Cell Line Name |
HEK293-Human IL-10 R alpha/IL-10 R beta (Luc)-Reporter Cell |
| Protein Family |
Interleukin receptor family |
| Gene |
IL-10 R alpha/IL-10 R beta |
| NCBI Gene ID |
IL - 10 R alpha: 3587
IL - 10 R beta: 3588 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (40 μg/mL) + Zeocin (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
