HEK293-Human IL-22 R alpha 1/IL-10 R beta (Luc)-Reporter Cell
| Catalog Number |
CBCL-0205 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell has been designed to express the STAT3 signaling response element, as well as the full-length human IL-22 R alpha 1 (Uniprot: Q8N6P7) and IL-10 R beta (Uniprot: Q08334) receptors. Stimulation with human IL-22 protein activates receptor-mediated signaling, driving STAT3-mediated luminescence. Neutralization of the biological effect of human IL-22 protein with corresponding antibodies decreases luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human IL-22 protein
Background
IL - 22 R alpha 1 and IL - 10 R beta form a receptor complex that binds interleukin - 22 (IL - 22). IL - 22 is involved in the regulation of immune responses at epithelial barriers, such as the skin and gut, and plays roles in tissue repair, host defense, and inflammation.
Product Information
| Category |
Cytokine Receptor Cell Lines |
| Cell Line Name |
HEK293-Human IL-22 R alpha 1/IL-10 R beta (Luc)-Reporter Cell |
| Protein Family |
Interleukin receptor family |
| Gene |
IL-22 R alpha 1/IL-10 R beta |
| NCBI Gene ID |
IL - 22 R alpha 1: 149556
IL - 10 R beta: 3588 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin (40 μg/mL) + Zeocin (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
