HEK293-Human IL-31 RA/OSMR (Luc)-Reporter Cell
| Catalog Number |
CBCL-0207 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human IL-31 RA/OSMR (Luc) HEK293 Reporter Cell has been developed to express the STAT3 signaling response element, as well as the full-length human IL-31 RA (Uniprot: Q8NI17-1) and OSMR (Uniprot: Q99650-1) receptors. Stimulation with human IL-31 protein activates the IL-31/IL-31 RA interaction, driving STAT3-mediated luminescence. Neutralization of the biological effect of human IL-31 protein with corresponding antibodies decreases luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human IL-31 protein.
Background
IL - 31 RA (Interleukin 31 Receptor Alpha) and OSMR (Oncostatin M Receptor) form a receptor complex that binds interleukin - 31 (IL - 31). IL - 31 is associated with skin inflammation and pruritus (itching), and the receptor complex mediates its biological effects in skin cells and immune cells.
Product Information
| Category |
Cytokine Receptor Cell Lines |
| Cell Line Name |
HEK293-Human IL-31 RA/OSMR (Luc)-Reporter Cell |
| Protein Family |
Interleukin receptor family |
| Gene |
IL-31 RA/OSMR |
| NCBI Gene ID |
IL - 31 RA: 386652
OSMR: 9420 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (40 μg/mL) + Zeocin (20μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
