HEK293-Human IL-7 R alpha/CD132 (Luc)-Reporter Cell
| Catalog Number |
CBCL-0210 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human IL-7 R alpha/CD132 (Luc) HEK293 Reporter Cell has been engineered to express the STAT5 signaling response element, as well as the full-length human IL-7 R alpha (Uniprot: P16871-1) and CD132 (Uniprot: P31785-1) receptors. Stimulation with human IL-7 protein activates the IL-7 R alpha/CD132 interaction, driving STAT5-mediated luminescence. Neutralization of the biological effect of human IL-7 protein with corresponding antibodies decreases luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human IL-7 protein
Background
IL - 7 R alpha (Interleukin 7 Receptor Alpha) combines with the common gamma chain (CD132) to form the interleukin - 7 receptor complex. IL - 7 is crucial for the development, survival, and proliferation of T cells and B cells, especially during early immune system development.
Product Information
| Category |
Cytokine Receptor Cell Lines |
| Cell Line Name |
HEK293-Human IL-7 R alpha/CD132 (Luc)-Reporter Cell |
| Protein Family |
Interleukin receptor family |
| Gene |
IL-7 R alpha/CD132 |
| NCBI Gene ID |
IL - 7 R alpha: 9021
CD132 (IL - 2 R gamma): 3561 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) + G418 (200 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
