HEK293-Human OX40 (Luc)-Reporter Cell

Introduction

The HEK293-Human OX40 (Luc)-Reporter Cell is engineered to express the NF-κB signaling response element and the receptor full-length human OX40 (Uniprot: P43489), which can drive luciferase expression systems by OX40 ligand/agonist antibody stimulation. When stimulated with human OX40 ligand protein, the OX40 ligand/OX40 interaction drives NF-κB-mediated luminescence. Inhibition of OX40 ligand binding to OX40 by either anti-OX40 ligand or anti-OX40 antibodies results in a decrease in luminescence.

Application

Screen for anti-human OX40 ligand or anti-human OX40 neutralizing antibody.

Background

OX40 (TNFRSF4, CD134) is a co-stimulatory receptor expressed on activated T cells. It binds to OX40 Ligand (TNFSF4) on APCs, enhancing T cell survival, proliferation, and cytokine production. OX40 agonists are investigated for cancer immunotherapy and autoimmune disease modulation.

Product Information