HEK293-Human PTH1R (Luc)-Reporter Cell
| Catalog Number |
CBCL-0258 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This HEK293 reporter cell line expresses the CREB signaling response element and the full-length human PTH1R receptor (Uniprot: Q03431). Luciferase expression is driven by PTH1R agonists or PTH stimulation. In the absence of agonist or PTH, the PTH1R receptor is not activated, and luminescence is low. In the presence of agonist or PTH, PTH1R pathway-activated luminescence can be detected in a dose-dependent manner.
Application
Screen for agonists that can bind and activate PTH1R
Background
PTH1R (Parathyroid Hormone 1 Receptor) is a G protein-coupled receptor that binds parathyroid hormone (PTH) and regulates calcium and phosphorus homeostasis.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human PTH1R (Luc)-Reporter Cell |
| Protein Family |
Parathyroid hormone receptor family |
| Gene |
PTH1R |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
