HEK293-Human TACI (Luc)-Reporter Cell
| Catalog Number |
CBCL-0261 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This HEK293 reporter cell line expresses the NF-κB signaling response element and the full-length human TACI receptor (Uniprot: O14836-1). Luciferase expression is driven by stimulation with human APRIL or BAFF protein. Neutralization of the biological effect of the ligand-receptor interaction by corresponding antibodies or fusion proteins results in decreased luminescence.
Application
Screen for neutralizing antibodies or fusion proteins blocking the stimulation of human APRIL or BAFF protein.
Background
TACI is a tumor necrosis factor receptor superfamily member that interacts with ligands such as BAFF and APRIL to regulate B cell development and antibody production.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human TACI (Luc)-Reporter Cell |
| Protein Family |
Tumor necrosis factor receptor superfamily |
| Gene |
TACI |
| NCBI Gene ID |
23495 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
