HEK293-Human TGF-beta R (Luc)-Reporter Cell
| Catalog Number |
CBCL-0215 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human TGF-beta R (Luc) HEK293 Reporter Cell was designed to express the Smad signaling response element driving luciferase expression. Stimulation with TGF-beta (TGF-beta 1/2/3) protein can trigger Smad-mediated luminescence. Neutralization of the ligand-receptor interaction by corresponding antibodies results in decreased luminescence.
Application
Screen for neutralizing antibodies blocking the ligand-receptor interaction.
Background
TGF - beta R (Transforming Growth Factor - beta Receptor) includes type I (TGF - beta R1) and type II (TGF - beta R2) receptors. They form a heteromeric receptor complex that binds transforming growth factor - beta (TGF - beta) cytokines. TGF - beta signaling is involved in cell growth, differentiation, apoptosis, and immune regulation, and plays important roles in development, tissue homeostasis, and disease processes.
Product Information
| Category |
Cytokine Receptor Cell Lines |
| Cell Line Name |
HEK293-Human TGF-beta R (Luc)-Reporter Cell |
| Protein Family |
Transforming growth factor beta receptor family |
| Gene |
TGF-beta R |
| NCBI Gene ID |
TGF - beta R1: 7040
TGF - beta R2: 7042 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
