HEK293-Human THRA (Luc)-Reporter Cell
| Catalog Number |
CBCL-0264 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This HEK293 reporter cell line expresses the thyroid hormone response element (TRE) and the full-length human THRA receptor (Uniprot: P10827-2). Luciferase expression is driven by thyroid hormone (TH) or thyroid hormone receptor (THR) agonists. In the absence of TH or THR agonists, the THRA receptor is not activated, and luminescence is low. In the presence of TH or THR agonists, THRA pathway-activated luminescence can be detected in a dose-dependent manner.
Application
Screen for THR-β-selective agonists.
Background
THRA (Thyroid Hormone Receptor Alpha) is a nuclear receptor that binds thyroid hormones and regulates gene expression involved in metabolism, growth, and development.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human THRA (Luc)-Reporter Cell |
| Protein Family |
Thyroid hormone receptor family |
| Gene |
THRA |
| NCBI Gene ID |
7067 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
