HEK293-Human TrkA (Luc)-Reporter Cell
| Catalog Number |
CBCL-0268 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This HEK293 reporter cell line expresses the SRE signaling response element and the full-length human TrkA receptor (Uniprot: P04629-1). When stimulated with human NGF protein, the NGF/TrkA interaction drives SRE-mediated luminescence. Neutralization of the biological effect of human NGF protein by corresponding antibodies results in decreased luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human NGF protein.
Background
TrkA is a receptor tyrosine kinase that binds nerve growth factor (NGF) and regulates neuronal survival, differentiation, and synapse formation.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human TrkA (Luc)-Reporter Cell |
| Protein Family |
Neurotrophic tyrosine kinase receptor family |
| Gene |
TrkA |
| NCBI Gene ID |
4914 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (40 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
