HEK293-Human TSHR (Luc)-Reporter Cell
| Catalog Number |
CBCL-0269 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This HEK293 reporter cell line has been designed to express both the CREB signaling response element and the full-length human TSHR receptor (Uniprot: P16473-1). Upon stimulation with human TSH protein or a TSHR agonist antibody, receptor-mediated signaling activates CREB-mediated luminescence. The biological effect of human TSH protein can be neutralized by a corresponding antibody, leading to a decrease in luminescence.
Application
Screen for anti-human TSHR agonist antibody.
Background
TSHR (Thyroid-Stimulating Hormone Receptor) is a G protein-coupled receptor that binds TSH and regulates thyroid hormone synthesis and secretion.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-Human TSHR (Luc)-Reporter Cell |
| Protein Family |
Thyroid-stimulating hormone receptor family |
| Gene |
TSHR |
| NCBI Gene ID |
7253 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
