HEK293-IGFβ/Activin A/Myostatin-Responsive-Luciferase-Reporter Cell Line

Introduction

A HEK293 cell line that expresses firefly luciferase under the control of SMAD-responsive elements (SMAD binding elements, SBE). This cell line monitors the activity of the TGFβ (transforming growth factor beta)/SMAD signaling pathway. The cell line has been validated by activation in response to human TGFβ1 and other cytokines of the TGFβ1 superfamily, such as Activin A, GDF-8/Myostatin, and GDF-11/BMP-11. Luciferase activity induced by human TGFβ1 was decreased by SB525334, an inhibitor of the TGFβ1-receptor. Luciferase activity induced by Activin A, GDF-8/Myostatin, and GDF-11/BMP-11 was decreased by Bimagrumab, an antibody of the ActRII. Activin A-induced luciferase activity was also decreased by Activin Blocker, an ActRIIA-Fc fusion protein, and an Anti-TGFβ antibody.

Application

Drug screening and biological assays

Background

If referring to IGF1R (Insulin - like Growth Factor 1 Receptor), it is a receptor tyrosine kinase that binds to IGF - 1 and IGF - 2 and mediates their effects on cell growth, proliferation, and differentiation. Activin A and Myostatin are part of the TGF - β superfamily with distinct roles in growth and development regulation.

Product Information