HEK293-mAChR-M5/NFAT (Luc) -Stable Cell Line
| Catalog Number |
CBCL-0735 |
| Price |
Online Inquiry |
| Unit Quantity |
2 x 10^6 cells / 1.0 ml |
Introduction
The HEK293-mAChR-M5/NFAT (Luc) -Stable Cell Line is a HEK293 cell line that stably expresses the full-length human muscarinic acetylcholine receptor M5 and a firefly luciferase reporter under the control of the NFAT response element. This cell line has been validated by flow cytometry and responds to muscarinic acetylcholine receptor agonists such as Oxotremorine M and Carbamoylcholine chloride.
Application
Drug screening and biological assays
Background
CHRM5 (Cholinergic Receptor Muscarinic 5) is a G - protein - coupled receptor that binds acetylcholine. It is expressed in various tissues including the central nervous system and is involved in regulating neurotransmission, smooth muscle contraction, and other physiological processes.
Product Information
| Category |
Cell Signaling Cell Lines |
| Cell Line Name |
HEK293-mAChR-M5/NFAT (Luc) -Stable Cell Line |
| Protein Family |
Muscarinic acetylcholine receptor family; Nuclear factor of activated T-cells family |
| Gene |
mAChR-M5; NFAT |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
90% FBS + 10% DMSO |
| Storage Method |
Liquid Nitrogen |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
