HEK293-PPARγ-GAL4-Luciferase-Reporter Cell Line
| Catalog Number |
CBCL-0655 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A HEK293 cell line that expresses firefly luciferase under the control of the GAL4 upstream activation sequence (UAS) and stably expresses the human peroxisome proliferator-activated receptor γ ligand binding domain (PPARγ LBD, amino acids 173-475) fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD). This system allows for the specific measurement of the effects of PPARγ agonists with minimal cross-reactivity from other nuclear receptors or PPAR isoforms.
Application
Drug screening and biological assays
Background
PPARγ (Peroxisome Proliferator-Activated Receptor Gamma) is a nuclear receptor that regulates gene expression involved in adipogenesis, lipid metabolism, and insulin sensitivity. It is a target for drugs used to treat type 2 diabetes and metabolic syndrome.
Product Information
| Category |
Metabolic Enzymes Cell Lines |
| Cell Line Name |
HEK293-PPARγ-GAL4-Luciferase-Reporter Cell Line |
| Protein Family |
Peroxisome proliferator-activated receptor family |
| Gene |
PPARγ |
| NCBI Gene ID |
5468 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Cell Freezing Medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
