HEK293-TCF/LEF (Luc)-Reporter Cell
| Catalog Number |
CBCL-0262 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This HEK293 reporter cell line expresses the TCF/LEF signaling response element driving luciferase expression, designed to monitor the activity of the Wnt/β-catenin signaling pathway. When stimulated with human Wnt protein, receptor-mediated signaling drives TCF/LEF-mediated luminescence. Inhibition of the biological effect of human Wnt protein by corresponding inhibitors results in decreased luminescence.
Application
Monitor Wnt signaling pathway activity.
Background
TCF/LEF (T-cell factor/lymphoid enhancer-binding factor) is a family of transcription factors that regulate gene expression in response to Wnt signaling, playing a role in development and cancer.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293-TCF/LEF (Luc)-Reporter Cell |
| Protein Family |
T-cell factor/lymphoid enhancer factor family |
| Gene |
TCF/LEF |
| NCBI Gene ID |
TCF7/6932 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
