HEK293-TCF/LEF-Reporter Cell Line (Wnt Signaling Pathway)
| Catalog Number |
CBCL-0725 |
| Price |
Online Inquiry |
| Unit Quantity |
2 x 10^6 cells / 1.0 ml |
Introduction
The HEK293-TCF/LEF-Reporter Cell Line (Wnt Signaling Pathway) is designed for monitoring the activity of the Wnt/b-catenin signaling pathway. It contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse Wnt3a and to the treatment with an inhibitor of Wnt/b-catenin signaling pathway.
Application
Drug screening and biological assays
Background
TCF7 (T - Cell Factor 7) is a member of the TCF/LEF family of transcription factors. These factors interact with β - catenin in the Wnt signaling pathway and regulate the expression of Wnt target genes, playing important roles in cell fate determination, tissue development, and cancer.
Product Information
| Category |
Cell Signaling Cell Lines |
| Cell Line Name |
HEK293-TCF/LEF-Reporter Cell Line (Wnt Signaling Pathway) |
| Protein Family |
T-cell factor/lymphoid enhancer factor family |
| Gene |
TCF/LEF |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
90% FBS + 10% DMSO |
| Storage Method |
Liquid Nitrogen |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
