HEK293T-SRF-RE-luc2-Stable Cell Line
| Catalog Number |
CBCL-0285 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
This stable HEK293T cell line expresses exogenous luciferase under the control of the RhoA signaling pathway, enabling studies of RhoA-dependent gene expression and signaling events.
Application
Drug screening and biological assays
Background
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE) in the promoter region of target genes. SRF regulates the expression of many immediate-early genes, such as c-Fos, and is involved in cell cycle regulation, cell growth, and cell differentiation. SRF is widely expressed in various tissues and plays a crucial role in developmental processes, including cardiac muscle cell differentiation and angiogenesis. The term "SRF-RE" likely refers to the SRE or SRF-responsive element, which is a DNA sequence that binds SRF and mediates its transcriptional activity.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
HEK293T-SRF-RE-luc2-Stable Cell Line |
| Protein Family |
Serum response factor-response element family |
| Gene |
SRF-RE |
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Hygromycin B |
| Recommended Medium |
DMEM+10%FBS+150µg/mL Hygromycin B |
| Freeze Medium |
70% DMEM+20% FBS+10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
