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HEK293T-Tet-on-GFP-Stable Cell Line

Catalog Number CBCL-0357
Price Online Inquiry
Unit Quantity 1 x 10^6 cells / 1.0 ml

Introduction

This stable HEK293T cell line expresses exogenous GFP under the control of a tetracycline-inducible (Tet-On) system, allowing for regulated expression studies and visualization of cellular processes.

Application

Drug screening and biological assays

Background

Tet-on is another system for controlling gene expression, where the presence of tetracycline or its analogs activates transcription of a target gene.

Product Information

Category Fluorescence Labeled Cell Lines
Cell Line Name HEK293T-Tet-on-GFP-Stable Cell Line
Protein Family Tetracycline-controlled transcriptional activation family
Gene Tet-on
Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Morphology Epithelial
Growth Properties Adherent
Selection Marker Puromycin
Recommended Medium DMEM+10%FBS+1µg/mL Puromycin
Freeze Medium 70% DMEM+20% FBS+10% DMSO
Storage Method Frozen in liquid nitrogen.
Stability Validated for at least 10 passages
Mycoplasma Negative
Sterility Testing Negative
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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