Human AKR1B1-Luc2 Stable NCl-H1975 Cell Line
| Catalog Number |
CBCL-340 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable NCI-H1975 cell line expressing exogenous human AKR1B1 gene and luciferase
Application
Drug screening and biological assays
Background
AKR1B1, also known as AR, is an enzyme that is encoded by the AKR1B1 gene in humans. Adapting AKR1B1 inhibitors could as well prevent sepsis complications, prevent angiogenesis, ameliorate mild or asymptomatic diabetic cardiovascular autonomic neuropathy and may be a promising strategy for the treatment of endotoxemia and other ROS-induced inflammatory diseases.
Product Information
| Species |
Human |
| Host Cell Line |
NCI-H1975 |
| Cell Type |
Human lung Adenocarcinoma cell line |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI1640 + 10% FBS + 1 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Epithelial |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
