Human-AKR1B1 Stable HCC827 Cell Line
| Catalog Number |
CBCL-254 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable HCC827 cell line expressing exogenous human AKR1B1 gene and luciferase.
Application
Drug screening and biological assays
Background
AKR1B1, also known as AR, is an enzyme that is encoded by the AKR1B1 gene in humans, which belongs to the aldehyde-keto reductase superfamily with a widely expression in human organs. Adapting AKR1B1 inhibitors could as well prevent sepsis complications and prevent angiogenesis.
Product Information
| Species |
Human |
| Host Cell Line |
HCC827 |
| Cell Type |
Human non-small cell lung cancer cell line |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Kinases |
| Recommended Medium |
RPMI1640 + 10% FBS + 2 μg/ml Puromycin |
| Selection Marker |
Puromycin |
| Morphology |
Epithelial |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
