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Human ARv7 Stable HEK293 Cell Line

Catalog Number CBCL-382
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HEK293 cell line expressing exogenous human ARv7 gene

Application

Drug screening and biological assays

Background

ARv7, also known as AR3. As the most common AR-V, the androgen receptor splice variant 7 (AR-V7), a splice variant of the androgen receptor mRNA resulting in the truncation of the ligand-binding domain, is a promising predictive tumor marker and a relevant selection marker for the treatment of advanced prostate cancer.

Product Information

Species Human
Host Cell Line HEK293
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 1 μg/ml Puromycin
Selection Marker Puromycin and hygromycin B
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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