Human B7H3 Stable 293T Cell Line

Catalog Number

CBCL-451

Price

Online Inquiry

Unit Quantity

1x10⁶ cells / 1.0 ml

Introduction

Stable HEK293T cell line expressing exogenous human B7H3 gene

Application

Drug screening and biological assays

Background

CD276, also named as human B7 homolog 3 (B7-H3), is a member of the B7 immune protein family. CD276 is expressed on dendritic cells, T cells, B cells, and NK cells, and play an important role immune cell activation as a co-stimulatory molecule.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human B7H3 Stable 293T Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Morphology	Monolayer
Growth Properties	Adherent
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 30 hours
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.