Human B7H3 Stable MC38 Cell Line

Catalog Number	CBCL-424
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable MC38 clone expressing exogenous human B7H3 gene

Application

Drug screening and biological assays

Background

CD276, also named as human B7 homolog 3 (B7-H3), is a member of the B7 immune protein family. CD276 is expressed on dendritic cells, T cells, B cells, and NK cells, and play an important role immune cell activation as a co-stimulatory molecule.

Product Information

Species	Mouse
Host Cell Line	MC38 cell line
Cell Type	Mouse colon cancer cell line
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	RPMI1640 + 10% FBS + 5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:10.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 20 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.