Human B7H4 Stable Ba/F3 Cell Line

Catalog Number	CBCL-266
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable Ba/F3 clone expressing exogenous human B7H4 gene

Application

Drug screening and biological assays

Background

B7H4, also known as VTCN1, is a protein that belongs to the B7 Immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	Human B7H4 Stable Ba/F3 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Mouse
Host Cell Line	Ba/F3
Cell Type	Pro-B cells
Morphology	Mostly round
Growth Properties	Suspension
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:6.
Doubling Time	Approximately 30 hours
Recommended Medium	RPMI1640 + 10% FBS + 1 μg/ml puromycin +8 ng/ml mIL-3
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.