Human CD123 Stable 293T Cell Line

Catalog Number	CBCL-475
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human CD123 gene

Application

Drug screening and biological assays

Background

CD123, also known as interleukin-3 receptor, belongs to the type I cytokine receptor family, and form a heterodimer with common beta (CD131) subunit, which transmit the signal after binding with interleukin-3. (CD123 is mainly found on pluripotent progenitor cells, and also many subtype of acute myeloid leukemia (AML), and is a potential target of treatment for AML.

Product Information

Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.