Human CD137 Stable 293T Cell Line

Catalog Number	CBCL-441
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous human CD137 gene

Application

Drug screening and biological assays

Background

CD137, also named 4-1BB ligand receptor and TNFRSF9, is a member of the tumor necrosis (TNF) receptor family and a famous co-stimulatory immune checkpoint molecule. CD137 is expressed on activated T cells, and nature killer cells, crosslinking of CD137 by agonistic antibody or its ligand enhance T cell proliferation, IL2 secretion and cytolytic activities.

Product Information

Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.